Material for ‘Two-Photon Time-Lapse Microscopy of BODIPY- Cholesterol Reveals Anomalous Sterol Diffusion in Chinese Hamster Ovary Cells’
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چکیده
Figure S 1: Chemical structure of BODIPY-cholesterol (BChol) and dehydroergosterol (DHE). A, structure of BODIPY-cholesterol with the fluorophore (green, with light green underlay to highlight the fluorescent group) at carbon 24 of the sterol side chain. B, structure of DHE. Differences to cholesterol are indicated in green and red; i.e., an extra methyl group and double bond in the alkyl side chain and two additional double bonds in the steroid ring system. Only one additional double bond in the second ring distinguishes DHE from ergosterol (indicated in red). The three conjugated double bonds in the steroid ring (indicated in light blue) give DHE its slight fluorescence.
منابع مشابه
Two-photon time-lapse microscopy of BODIPY-cholesterol reveals anomalous sterol diffusion in chinese hamster ovary cells
UNLABELLED BACKGROUND Cholesterol is an important membrane component, but our knowledge about its transport in cells is sparse. Previous imaging studies using dehydroergosterol (DHE), an intrinsically fluorescent sterol from yeast, have established that vesicular and non-vesicular transport modes contribute to sterol trafficking from the plasma membrane. Significant photobleaching, however, ...
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